Fabricating brightly fluorescent layers with nanometric thickness and digitally controlled lateral structuration remains a challenge for next-generation photonic devices, optical calibration standards, and biocompatible interfaces. Here, we introduce Nano-Bead Emitters (NBEs), hydrogel nanoparticles covalently functionalized with fluorophores, as a universal, water-processable ink platform for fabricating programmable nanometric fluorescent architectures. By immobilizing fluorophores within a charged nanohydrogel scaffold, the platform entirely decouples film morphology from dye solubility. This molecule-independent strategy enables spectrally distinct, inherently water-insoluble dyes to be processed using a single, standardized aqueous ink formulation. Combined with laser-induced forward transfer (LIFT) printing, this additive approach yields highly uniform fluorescent layers (~7 nm thickness, sub-nanometric roughness). This structural invariance produces complex multicolor patterns sharing identical thickness and surface morphology across all spectral channels, a critical requirement for quantitative optical calibration. Furthermore, LIFT printing provides programmable, layer-by-layer control over fluorescence intensity via successive deposition cycles, yielding precisely tunable brightness without aggregation-caused quenching. This maskless technique enables rapid, high-fidelity printing of both monochromatic and multicolor patterns over macroscopic areas with absolute spatial resolution. Finally, these universally compatible NBE inks stably deposit onto diverse substrates (glass, polymers, semiconductors, metasurfaces), effectively bridging scalable manufacturing with high-performance integrated photonic systems.
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